文章基本信息

标题：Protective Effects Induced by a Hydroalcoholic Allium sativum Extract in Isolated Mouse Heart
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作者：Lucia Recinella ; Annalisa Chiavaroli ; Fabrizio Masciulli 等
期刊名称：Nutrients
电子版ISSN：2072-6643
出版年度：2021
卷号：13
期号：7
页码：2332
DOI：10.3390/nu13072332
出版社：MDPI Publishing
摘要：The aim of the present study was to investigate the possible protective effects of a garlic hydroalcoholic extract on the burden of oxidative stress and inflammation occurring on mouse heart specimens exposed to E. coli lipopolysaccharide (LPS), which is a well-established inflammatory stimulus. Headspace solid-phase microextraction combined with the gas chromatography–mass spectrometry (HS-SPME/GC–MS) technique was applied to determine the volatile fraction of the garlic powder, and the HS-SPME conditions were optimized for each of the most representative classes of compounds. CIEL*a*b* colorimetric analyses were performed on the powder sample at the time of delivery, after four and after eight months of storage at room temperature in the dark, to evaluate the color changing. Freshly prepared hydroalcoholic extract was also evaluated in its color character. Furthermore, the hydroalcoholic extract was analyzed through GC–MS. The extract was found to be able to significantly inhibit LPS-induced prostaglandin (PG) E2 and 8-iso-PGF2α levels, as well as mRNA levels of cyclooxygenase (COX)-2, interleukin (IL)-6, and nuclear factor-kB (NF-kB), in heart specimens. Concluding, our findings showed that the garlic hydroalcoholic extract exhibited cardioprotective effects on multiple inflammatory and oxidative stress pathways.
关键词：garlic; multimethodological evaluation; CIEL*a*b*; HS-SPME/GC–MS; oxidative stress; inflammation; bioinformatics garlic ; multimethodological evaluation ; CIEL*a*b* ; HS-SPME/GC–MS ; oxidative stress ; inflammation ; bioinformatics