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标题：Characterization of crude 5’-phosphodiesterase from germinated adzuki (Vigna angularis L.) beans
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作者：Liew Phing Pui ; Abdulkarim Sabo Mohammed ; Hasanah Mohd Ghazali 等
期刊名称：Acta Scientarum Polonorum - Technologia Alimentaria
印刷版ISSN：1644-0730
电子版ISSN：1898-9594
出版年度：2020
卷号：19
期号：3
页码：319-331
DOI：10.17306/J.AFS.0804
出版社：Agricultural University of Poznan Press
摘要：BACKGROUND :5'-Phosphodiesterase (5'-PDE) is an enzyme that hydrolyzes RNA to form 5'-inosine monophosphate (5'-IMP) and 5'-guanosine monophosphate (5'-GMP). These 5'-nucleotides can function as flavor enhancers. Adzuki beans (Vigna angularis L.) are found to be high in 5'-PDE. METHODS :5'-phosphodiesterase (5'-PDE) enzyme was characterized from adzuki beans, in which the optimum pH and temperature were determined. In addition, the stability of 5'-PDE was assessed at different pH and temperature. The effects of cations and EDTA were evaluated to characterize the 5'-PDE enzymes further. RESULTS :The alkaline 5'-phosphodiesterase has an optimum pH of 8.5. This enzyme is also thermostable, with an optimum temperature of 80°C. The stability in terms of temperature and pH was also determined, and was found to be stable in the pH range of 7.0-8.5. This enzyme was found to retain more than 80% of its activity for 4 days at 60 and 65°C. In addition, the effects of 14 different metal ions, 4 types of detergents and ethylenediaminetetraacetic acid (EDTA) on 5'-PDE were studied. Ca2 , K , Mg2 and Li activated 5'-PDE while Na , Zn2 , Ni , Hg , Cu2 , Pb2 , Fe2 , Al3 , Ba2 and Co2 were inhibitory. EDTA, Triton X-100 and sodium dodecyl sulfate (SDS) were strong inhibitors of 5'-PDE, while Tween 80 and Tween 20 were slightly inhibitory. The effects of cations and EDTA suggest that 5'-PDE from adzuki beans is a metalloenzyme. CONCLUSIONS :Although 5'-PDE from adzuki beans has a high temperature optimum of 80°C, the enzyme is more stable at 60°C, and different cations affected the activity of the enzyme differently.
关键词：5’-phosphodiesterase;cations;detergents;optimum pH;thermostability
其他关键词：5’-phosphodiesterase, thermostability, optimum pH, detergents, cations