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标题：Preparative separation of three isoquinoline alkaloids from Berberidis radix by pH-zone-refining countercurrent chromatography
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作者：Changlei Sun ; Wei Zhao ; Wenjuan Duan 等
期刊名称：Chemical Industry and Chemical Engineering Quarterly
印刷版ISSN：1451-9372
出版年度：2018
卷号：24
期号：1
页码：1-7
DOI：10.2298/CICEQ151220041S
出版社：Association of the Chemical Engineers
摘要：Berberidis radix, as a traditional Chinese herbal medicine, is well known as the main source of berberine. This herbal medicine has been used for the treatment of many diseases and alkaloids including jatrorrhizine, palmatine, and berberine are major bioactive constituents. Few literatures report the preparative separation of these alkaloids from Berberidis radix. In the present study, the combinative application of ultrasonic extraction and pH-zone-refining countercurrent chromatography was used to extract and purify these alkaloids. The PZRCCC separation was performed in a two-phase solvent system composed of chloroform– methanol–water (4:3:3 volume ratio), where 40 mM HCl was added in the upper aqueous stationary phase as a retainer and 10 mM triethylamine was added in the lower organic mobile phase as an eluter. From a crude sample of 1.5 g, palmatine (251.6 mg), berberine (392.5 mg), and jatrorrhizine (412.6 mg) were obtained in a single run with purities of all being over 93.0%, analyzed by HPLC. The chemical structures of the separated alkaloids were identified by electrospray ionization–mass spectrometry (ESI-MS), 1H-NMR and 13C-NMR.
关键词：separation; palmatine; berberine; jatrorrhizine; Berberidis radix; pH-zone-refining countercurrent chromatography