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标题：UCP1 and TRPM8 Expression in the Brown Fat Did Not Affect the Restriction of Menthol-Induced Hyperthermia by Estradiol in Ovariectomized Rats
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作者：Yuki UCHIDA ; Izumi SATO ; Koyuki ATSUMI 等
期刊名称：Journal of Nutritional Science and Vitaminology
印刷版ISSN：0301-4800
电子版ISSN：1881-7742
出版年度：2021
卷号：67
期号：2
页码：130-134
DOI：10.3177/jnsv.67.130
出版社：Center for Academic Publications Japan
摘要：Estradiol (E 2 ) modulates the central and peripheral thermoregulatory responses to cold. Menthol is an agonist of transient receptor potential melastatin type 8 (TRPM8), which is a peripheral cold receptor. E 2 suppresses menthol-induced elevation of body temperature (T b ) in ovariectomized rats, but the mechanism is unknown. The aim of the present study is to investigate the effect of E 2 on uncoupling protein 1 (UCP1), a thermogenic gene, and TRPM8 mRNA levels in ovariectomized rats applied menthol. A silastic tube was implanted in ovariectomized rats with and without E 2 underneath the dorsal skin (E 2 ( ) and E 2 (−) groups), and data loggers for T b measurement into peritoneal cavity. After application of 10% L-menthol or vehicle to the skin of the whole trunk of rats, T b was measured for 2 h. The interscapular brown adipose tissue (BAT) and spinal ganglia of cervical, thoracic, and lumbar parts were obtained for RT-qPCR assay. In the menthol application, T b in the E 2 ( ) group was lower than that in the E 2 (−) group. The UCP1 mRNA in the BAT, TRPM8 mRNA in the BAT and spinal ganglia in all areas did not differ between the E 2 ( ) and E 2 (−) groups. In conclusion, the UCP1 and TRPM8 expression in the brown fat did not affect the restriction of the menthol-induced hyperthermia by estradiol in ovariectomized rats.
关键词：menthol;UCP1;TRPM8;brown adipose tissue;estradiol