文章基本信息

标题：Exosomes Derived from Fisetin-Treated Keratinocytes Mediate Hair Growth Promotion
本地全文：下载
作者：Mizuki Ogawa ; Miyako Udono ; Kiichiro Teruya 等
期刊名称：Nutrients
电子版ISSN：2072-6643
出版年度：2021
卷号：13
期号：6
页码：2087
DOI：10.3390/nu13062087
出版社：MDPI Publishing
摘要：Enhanced telomerase reverse transcriptase (TERT) levels in dermal keratinocytes can serve as a novel target for hair growth promotion. Previously, we identified fisetin using a system for screening food components that can activate the TERT promoter in HaCaT cells (keratinocytes). In the present study, we aimed to clarify the molecular basis of fisetin-induced hair growth promotion in mice. To this end, the dorsal skin of mice was treated with fisetin, and hair growth was evaluated 12 days after treatment. Histochemical analyses of fisetin-treated skin samples and HaCaT cells were performed to observe the effects of fisetin. The results showed that fisetin activated HaCaT cells by regulating the expression of various genes related to epidermogenesis, cell proliferation, hair follicle regulation, and hair cycle regulation. In addition, fisetin induced the secretion of exosomes from HaCaT cells, which activated β-catenin and mitochondria in hair follicle stem cells (HFSCs) and induced their proliferation. Moreover, these results revealed the existence of exosomes as the molecular basis of keratinocyte-HFSC interaction and showed that fisetin, along with its effects on keratinocytes, caused exosome secretion, thereby activating HFSCs. This is the first study to show that keratinocyte-derived exosomes can activate HFSCs and consequently induce hair growth.
关键词：telomerase reverse transcriptase; keratinocyte–hair follicle stem cell interaction; exosomes; telogen–anagen transition; hair cycle regulation telomerase reverse transcriptase ; keratinocyte–hair follicle stem cell interaction ; exosomes ; telogen–anagen transition ; hair cycle regulation