文章基本信息

标题：Human Milk Oligosaccharide Profiles over 12 Months of Lactation: The Ulm SPATZ Health Study
本地全文：下载
作者：Linda P. Siziba ; Marko Mank ; Bernd Stahl 等
期刊名称：Nutrients
电子版ISSN：2072-6643
出版年度：2021
卷号：13
期号：6
页码：1973
DOI：10.3390/nu13061973
出版社：MDPI Publishing
摘要：Human milk oligosaccharides (HMOs) have specific dose-dependent effects on child health outcomes. The HMO profile differs across mothers and is largely dependent on gene expression of specific transferase enzymes in the lactocytes. This study investigated the trajectories of absolute HMO concentrations at three time points during lactation, using a more accurate, robust, and extensively validated method for HMO quantification. We analyzed human milk sampled at 6 weeks (n = 682), 6 months (n = 448), and 12 months (n = 73) of lactation in a birth cohort study conducted in south Germany, using label-free targeted liquid chromatography mass spectrometry (LC-MS2). We assessed trajectories of HMO concentrations over time and used linear mixed models to explore the effect of secretor status and milk group on these trajectories. Generalized linear model-based analysis was used to examine associations between HMOs measured at 6 weeks of lactation and maternal characteristics. Results: Overall, 74%, 18%, 7%, and 1% of human milk samples were attributed to milk groups I, II, III, and IV, respectively. Most HMO concentrations declined over lactation, but some increased. Cross-sectionally, HMOs presented high variations within milk groups and secretor groups. The trajectories of HMO concentrations during lactation were largely attributed to the milk group and secretor status. None of the other maternal characteristics were associated with the HMO concentrations. The observed changes in the HMO concentrations at different time points during lactation and variations of HMOs between milk groups warrant further investigation of their potential impact on child health outcomes. These results will aid in the evaluation and determination of adequate nutrient intakes, as well as further (or future) investigation of the dose-dependent impact of these biological components on infant and child health outcomes.
关键词：human milk oligosaccharides (HMOs); absolute quantitation; most abundant HMOs (trioses to hexaoses); targeted LC-MS/MS; stages of lactation; human milk groups; maternal secretor and Lewis (Le/Se) status human milk oligosaccharides (HMOs) ; absolute quantitation ; most abundant HMOs (trioses to hexaoses) ; targeted LC-MS/MS ; stages of lactation ; human milk groups ; maternal secretor and Lewis (Le/Se) status