文章基本信息

标题：Structural characterization of PaFkbA: A periplasmic chaperone from Pseudomonas aeruginosa
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作者：Qin Huang ; Jing Yang ; Changcheng Li 等
期刊名称：Computational and Structural Biotechnology Journal
印刷版ISSN：2001-0370
出版年度：2021
卷号：19
页码：2460-2467
DOI：10.1016/j.csbj.2021.04.045
出版社：Computational and Structural Biotechnology Journal
摘要：Bacterial Mip-like FK506-binding proteins (FKBPs) mostly exhibit peptidyl–prolyl-cis/ trans -isomerase (PPIase) and chaperone activities. These activities are associated with various intracellular functions with diverse molecular mechanisms. Herein, we report the PA3262 gene-encoded crystal structure of the Pseudomonas aeruginosa PAO1′s Mip-like protein PaFkbA. Biochemical characterization of PaFkbA demonstrated PaFkbA’s chaperone activity for periplasmic protein MucD, a negative regulator of alginate biosynthesis. Furthermore, structural analysis of PaFkbA was used to describe the key features of PaFkbA chaperone activity. The outcomes of this analysis showed that the hinge region in the connecting helix of PaFbkA leads to the crucial conformational state transition for PaFkbA activity. Besides, the N-terminal domains participated in dimerization, and revealed its potential connection with FKBP domain and substrate binding. Mutagenesis and chaperone activity assay supported the theory that inter-domain motions are essential for PaFkbA function. These results provide biochemical and structural insights into the mechanism for FKBP’s chaperone activity and establish a plausible correlation between PaFkbA and P. aeruginosa MucD.
关键词：FKBP-type protein ; Chaperone activity ; Crystal structure ; Inter-domain rearrangement ; Pseudomonas aeruginosa