文章基本信息

标题：Mini review: Enzyme-based DNA synthesis and selective retrieval for data storage
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作者：Eojin Yoo ; Donghui Choe ; Jongoh Shin 等
期刊名称：Computational and Structural Biotechnology Journal
印刷版ISSN：2001-0370
出版年度：2021
卷号：19
页码：2468-2476
DOI：10.1016/j.csbj.2021.04.057
出版社：Computational and Structural Biotechnology Journal
摘要：The market for using and storing digital data is growing, with DNA synthesis emerging as an efficient way to store massive amounts of data. Storing information in DNA mainly consists of two steps: data writing and reading. The writing step requires encoding data in DNA, building one nucleotide at a time as a form of single-stranded DNA (ssDNA). Once the data needs to be read, the target DNA is selectively retrieved and sequenced, which will also be in the form of an ssDNA. Recently, enzyme-based DNA synthesis is emerging as a new method to be a breakthrough on behalf of decades-old chemical synthesis. A few enzymatic methods have been presented for data memory, including the use of terminal deoxynucleotidyl transferase. Besides, enzyme-based amplification or denaturation of the target strand into ssDNA provides selective access to the desired dataset. In this review, we summarize diverse enzymatic methods for either synthesizing ssDNA or retrieving the data-containing DNA.
关键词：Enzymatic DNA synthesis ; DNA data storage ; Single-stranded DNA ; Terminal deoxynucleotidyl transferase ; Synthetic Biology