文章基本信息

标题：The repertoire of ABC proteins in Clostridioides difficile
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作者：Methinee Pipatthana ; Phurt Harnvoravongchai ; Pisut Pongchaikul 等
期刊名称：Computational and Structural Biotechnology Journal
印刷版ISSN：2001-0370
出版年度：2021
卷号：19
页码：2905-2920
DOI：10.1016/j.csbj.2021.05.012
出版社：Computational and Structural Biotechnology Journal
摘要：ATP-binding cassette (ABC) transporters belong to one of the largest membrane protein superfamilies, which function in translocating substrates across biological membranes using energy from ATP hydrolysis. Currently, the classification of ABC transporters in Clostridioides difficile is not complete. Therefore, the sequence-function relationship of all ABC proteins encoded within the C. difficile genome was analyzed. Identification of protein domains associated with the ABC system in the C. difficile 630 reference genome revealed 226 domains: 97 nucleotide-binding domains (NBDs), 98 transmembrane domains (TMDs), 30 substrate-binding domains (SBDs), and one domain with features of an adaptor protein. Gene organization and transcriptional unit analyses indicated the presence of 78 ABC systems comprising 28 importers and 50 exporters. Based on NBD sequence similarity, ABC transporters were classified into 12 sub-families according to their substrates. Interestingly, all ABC exporters, accounting for 64% of the total ABC systems, are involved in antibiotic resistance. Based on analysis of ABC proteins from 49 C. difficile strains, the majority of core NBDs are predicted to be involved in multidrug resistance systems, consistent with the ability of this organism to survive exposure to an array of antibiotics. Our findings herein provide another step toward a better understanding of the function and evolutionary relationships of ABC proteins in this pathogen.
关键词：ATP-binding cassette transporter ; Active transporter ; In silico analysis ; ABC classification ; Antimicrobial resistance ; ABC transporter/protein ATP-binding cassette transporter/protein ; NBD Nucleotide-binding domain ; TMD Transmembrane domain ; SBD Substrate-binding domain ; NP Nucleotide-binding domain (NBD)-containing protein ; TP Transmembrane protein ; SP Substrate-binding domain (SBD)-containing protein ; ECF-type transporter Energy coupling factor-type transporter