文章基本信息

标题：Optimum DNA Extraction Methods for Edible Bird’s Nest Identification Using Simple Additive Weighting Technique
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作者：Meei Chien Quek ; Nyuk Ling Chin ; Sheau Wei Tan 等
期刊名称：Foods
电子版ISSN：2304-8158
出版年度：2021
卷号：10
期号：5
页码：1086
DOI：10.3390/foods10051086
出版社：MDPI Publishing
摘要：A simple additive weighting (SAW) technique was used to determine and compare the overall performance of five DNA extraction methods from conventional (SDS method) to commercial kits (Qiagen, Wizard, and NucleoSpin) for identifying origins of edible bird’s nest (EBN) using end-point polymerase chain reaction (PCR). A hybrid method (SDS/Qiagen) which has been developed by combining the conventional SDS method with commercialised Qiagen was determined as the most suitable in terms of speed and cost-effectiveness. The determination of optimum extraction method was by the performances on efficiency and feasibility, extracted DNA concentration, purity, PCR amplifiability, handling time and safety of reagents used. The hybrid SDS/Qiagen method is less costly compared to the commercial kits and offered a more rapid alternative to the conventional SDS method with significant improvement in the yield, purity and PCR amplifiability. The developed hybrid SDS/Qiagen method provides a more practical alternative over the lengthy process using conventional method and expensive process using commercial kits. Using the simple additive weighting (SAW) technique and analysis, the Qiagen method is considered the most efficient and feasible method without consideration of cost as it yielded the purest extracted DNA and achieved the highest PCR amplifiability with the shortest turnaround time.
关键词：SDS method; Qiagen method; polymerase chain reaction (PCR); multiple attribute decision making (MADM) analysis; Aerodramus SDS method ; Qiagen method ; polymerase chain reaction (PCR) ; multiple attribute decision making (MADM) analysis ; Aerodramus