文章基本信息

标题：N-glycome and N-glycoproteome of a hematophagous parasitic nematode Haemonchus
本地全文：下载
作者：Chunqun Wang ; Wenjie Gao ; Shi Yan 等
期刊名称：Computational and Structural Biotechnology Journal
印刷版ISSN：2001-0370
出版年度：2021
卷号：19
页码：2486-2496
DOI：10.1016/j.csbj.2021.04.038
出版社：Computational and Structural Biotechnology Journal
摘要：N-glycosylation is a physiologically vital post-translational modification of proteins in eukaryotic organisms. Initial work on Haemonchus contortus – a blood-sucking nematode of ruminants with a broad geographical distribution – has shown that this parasite harbors N-glycans with exclusive chitobiose modifications. Besides, several immunogenic proteins ( e.g. , amino- and metallo-peptidases) are known to be N-glycosylated in adult worms. However, an informative atlas of N-glycosylation in H. contortus is not yet available. Herein, we report 291 N-glycosylated proteins with a total of 425 modification sites in the parasite. Among them, many peptidase families ( e.g. , peptidase C1 and M1) including potential vaccine targets were enriched. Notably, the glycan-rich conjugates are distributed primarily in the intestine and gonads of adult worms, and consequently hidden from the host’s immune system. Collectively, these data provide a comprehensive atlas of N-glycosylation in a prevalent parasitic nematode while underlining its significance for infection, immunity and prevention.
关键词：Haemonchus contortus ; N-glycosylation ; N-glycan ; Mass spectrometry ; Glycopeptide