文章基本信息

标题：Sciatic nerve-conditioned medium with the addition of Croton blanchetianus Baill essential oil promotes morphological plasticity in spinal cord cultured cells
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作者：Luciana Cristina Borges Fernandes ; Salvador Viana Gomes Junior ; Ianara Mendonça da Costa 等
期刊名称：Research, Society and Development
电子版ISSN：2525-3409
出版年度：2021
卷号：10
期号：5
页码：1-12
DOI：10.33448/rsd-v10i5.14591
出版社：Grupo de Pesquisa Metodologias em Ensino e Aprendizagem em Ciências
摘要：Spinal cord injury is a devastating condition that leads to significant disabilities. The treatment for this problem is a challenge in neuroscience, and it is necessary to combine different strategies to obtain functional recovery outcomes. There are many herbal natural products, such as Croton blanchetianus Baill (CB) essential oil, a Brazilian semi-arid bush with neuroprotective substances tested in regeneration processes and synaptic plasticity. Thus, this study analyzed the cellular plasticity of spinal cord neurons and glial cells in the presence of sciatic nerve-conditioned medium (SNCM) before the addition of CB essential oil. Cell morphology was assessed over 96 hours, and immunocytochemistry analyses were conducted for GFAP, GAP-43 and NeuN. Photomicrographs were made by scanning electron microscopy (SEM). Morphological analysis showed evident trophic development in the groups that received CB essential oil (P>0.000), immunoreactivity for GFAP, GAP-43 and NeuN and the plasticity of these cells were confirmed by SEM. This pioneer study about the plasticity of spinal cord neurons and glial cells opens new possibilities and techniques with essential oils for cell therapy in the presence of SNCM, which promoted neuroprotective action.