文章基本信息

标题：Raman Microspectroscopic Analysis of Selenium Bioaccumulation by Green Alga Chlorella vulgaris
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作者：Martin Kizovský ; Zdeněk Pilát ; Mykola Mylenko 等
期刊名称：Biosensors
电子版ISSN：2079-6374
出版年度：2021
卷号：11
期号：4
页码：115
DOI：10.3390/bios11040115
出版社：MDPI Publishing
摘要：Selenium (Se) is an element with many commercial applications as well as an essential micronutrient. Dietary Se has antioxidant properties and it is known to play a role in cancer prevention. However, the general population often suffers from Se deficiency. Green algae, such as Chlorella vulgaris, cultivated in Se-enriched environment may be used as a food supplement to provide adequate levels of Se. We used Raman microspectroscopy (RS) for fast, reliable, and non-destructive measurement of Se concentration in living algal cells. We employed inductively coupled plasma-mass spectrometry as a reference method to RS and we found a substantial correlation between the Raman signal intensity at 252 cm−1 and total Se concentration in the studied cells. We used RS to assess the uptake of Se by living and inactivated algae and demonstrated the necessity of active cellular transport for Se accumulation. Additionally, we observed the intracellular Se being transformed into an insoluble elemental form, which we further supported by the energy-dispersive X-ray spectroscopy imaging.
关键词：selenium; algae; Raman spectroscopy; EDX; ICP-MS; bioaccumulation; Chlorella vulgaris selenium ; algae ; Raman spectroscopy ; EDX ; ICP-MS ; bioaccumulation ; Chlorella vulgaris