文章基本信息

标题：Early-stage dynamics of chloride ion–pumping rhodopsin revealed by a femtosecond X-ray laser
本地全文：下载
作者：Ji-Hye Yun ; Xuanxuan Li ; Jianing Yue 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2021
卷号：118
期号：13
页码：1
DOI：10.1073/pnas.2020486118
出版社：The National Academy of Sciences of the United States of America
摘要：Chloride ion–pumping rhodopsin (ClR) in some marine bacteria utilizes light energy to actively transport Cl − into cells. How the ClR initiates the transport is elusive. Here, we show the dynamics of ion transport observed with time-resolved serial femtosecond (fs) crystallography using the Linac Coherent Light Source. X-ray pulses captured structural changes in ClR upon flash illumination with a 550 nm fs-pumping laser. High-resolution structures for five time points (dark to 100 ps after flashing) reveal complex and coordinated dynamics comprising retinal isomerization, water molecule rearrangement, and conformational changes of various residues. Combining data from time-resolved spectroscopy experiments and molecular dynamics simulations, this study reveals that the chloride ion close to the Schiff base undergoes a dissociation–diffusion process upon light-triggered retinal isomerization.
关键词：time-resolved crystallography ; light-driven chloride-pumping rhodopsin ; X-ray free-electron laser ; serial femtosecond crystallography