文章基本信息

标题：Active forces shape the metaphase spindle through a mechanical instability
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作者：David Oriola ; Frank Jülicher ; Jan Brugués 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2020
卷号：117
期号：28
页码：16154-16159
DOI：10.1073/pnas.2002446117
出版社：The National Academy of Sciences of the United States of America
摘要：The metaphase spindle is a dynamic structure orchestrating chromosome segregation during cell division. Recently, soft matter approaches have shown that the spindle behaves as an active liquid crystal. Still, it remains unclear how active force generation contributes to its characteristic spindle-like shape. Here we combine theory and experiments to show that molecular motor-driven forces shape the structure through a barreling-type instability. We test our physical model by titrating dynein activity in Xenopus egg extract spindles and quantifying the shape and microtubule orientation. We conclude that spindles are shaped by the interplay between surface tension, nematic elasticity, and motor-driven active forces. Our study reveals how motor proteins can mold liquid crystalline droplets and has implications for the design of active soft materials.
关键词：mitotic spindle ; Xenopus laevis ; dynein ; liquid crystals ; active matter