文章基本信息

标题：Reply to Verwilt et al.: Experimental evidence against DNA contamination in SILVER-seq
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作者：Zixu Zhou ; Qiuyang Wu ; Zhangming Yan 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2020
卷号：117
期号：32
页码：18937-18938
DOI：10.1073/pnas.2008585117
出版社：The National Academy of Sciences of the United States of America
摘要：To evaluate any possible DNA contamination in Small Input Liquid Volume Extracellular RNA Sequencing (SILVER-seq), we carried out three types of tests. First, we tested the effectiveness of SILVER-seq’s DNase treatment step. Without DNase treatment, five of the five tested serum samples exhibited detectable cell-free DNA (cfDNA; Fig. 1 A ). After SILVER-seq’s DNase treatment step, none of the serum samples exhibited any detectable cfDNA (Fig. 1 A ), suggesting that SILVER-seq’s DNase treatment step thoroughly digested cfDNA in human serum samples.