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标题：Dynamic structural order of a low-complexity domain facilitates assembly of intermediate filaments
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作者：Vasiliy O. Sysoev ; Masato Kato ; Lillian Sutherland 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2020
卷号：117
期号：38
页码：23510-23518
DOI：10.1073/pnas.2010000117
出版社：The National Academy of Sciences of the United States of America
摘要：The coiled-coil domains of intermediate filament (IF) proteins are flanked by regions of low sequence complexity. Whereas IF coiled-coil domains assume dimeric and tetrameric conformations on their own, maturation of eight tetramers into cylindrical IFs is dependent on either “head” or “tail” domains of low sequence complexity. Here we confirm that the tail domain required for assembly of Drosophila Tm1-I/C IFs functions by forming labile cross-β interactions. These interactions are seen in polymers made from the tail domain alone, as well as in assembled IFs formed by the intact Tm1-I/C protein. The ability to visualize such interactions in situ within the context of a discrete cellular assembly lends support to the concept that equivalent interactions may be used in organizing other dynamic aspects of cell morphology.
关键词：intermediate filaments ; cross-beta polymerization ; low-complexity proteins ; solid-state NMR