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标题：Studies of lincosamide formation complete the biosynthetic pathway for lincomycin A
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作者：Shao-An Wang ; Chia-I Lin ; Jiawei Zhang 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2020
卷号：117
期号：40
页码：24794-24801
DOI：10.1073/pnas.2009306117
出版社：The National Academy of Sciences of the United States of America
摘要：The structure of lincomycin A consists of the unusual eight-carbon thiosugar core methyllincosamide (MTL) decorated with a pendent N -methylprolinyl moiety. Previous studies on MTL biosynthesis have suggested GDP-ᴅ- erythro -α-ᴅ- gluco -octose and GDP-ᴅ-α-ᴅ-lincosamide as key intermediates in the pathway. However, the enzyme-catalyzed reactions resulting in the conversion of GDP-ᴅ- erythro -α-ᴅ- gluco -octose to GDP-ᴅ-α-ᴅ-lincosamide have not yet been elucidated. Herein, a biosynthetic subpathway involving the activities of four enzymes—LmbM, LmbL, CcbZ, and CcbS (the LmbZ and LmbS equivalents in the closely related celesticetin pathway)—is reported. These enzymes catalyze the previously unknown biosynthetic steps including 6-epimerization, 6,8-dehydration, 4-epimerization, and 6-transamination that convert GDP-ᴅ- erythro -α-ᴅ- gluco -octose to GDP-ᴅ-α-ᴅ-lincosamide. Identification of these reactions completes the description of the entire lincomycin biosynthetic pathway. This work is significant since it not only resolves the missing link in octose core assembly of a thiosugar-containing natural product but also showcases the sophistication in catalytic logic of enzymes involved in carbohydrate transformations.
关键词：lincomycin ; biosynthesis ; lincosamide ; celesticetin ; thiosugar