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标题：Analysis of microbial diversity and functional differences in different types of high‐temperature Daqu
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作者：Yurong Wang ; Wenchao Cai ; Wenping Wang 等
期刊名称：Food Science & Nutrition
电子版ISSN：2048-7177
出版年度：2021
卷号：9
期号：2
页码：1003-1016
DOI：10.1002/fsn3.2068
出版社：John Wiley & Sons, Ltd.
摘要：Bacterial communities that enrich in high‐temperature Daqu are important for the Chinese maotai‐flavor liquor brewing process. However, the bacterial communities in three different types of high‐temperature Daqu (white Daqu, black Daqu, and yellow Daqu) are still undercharacterized. In this study, the bacterial diversity of three different types of high‐temperature Daqu was investigated using Illumina MiSeq high‐throughput sequencing. The bacterial community of high‐temperature Daqu is mainly composed of thermophilic bacteria, and seven bacterial phyla along with 262 bacterial genera were identified in all 30 high‐temperature Daqu samples. Firmicutes, Actinobacteria, Proteobacteria, and Acidobacteria were the dominant bacterial phyla in high‐temperature Daqu samples, while Thermoactinomyces , Staphylococcus , Lentibacillus , Bacillus , Kroppenstedtia , Saccharopolyspora , Streptomyces, and Brevibacterium were the dominant bacterial genera. The bacterial community structure of three different types of high‐temperature Daqu was significantly different ( p < .05). In addition, the results of microbiome phenotype prediction by BugBase and bacterial functional potential prediction using PICRUSt show that bacteria from different types of high‐temperature Daqu have similar functions as well as phenotypes, and bacteria in high‐temperature Daqu have vigorous metabolism in the transport and decomposition of amino acids and carbohydrates. These results offer a reference for the comprehensive understanding of bacterial diversity of high‐temperature Daqu.
关键词：bacterial diversity;Chinese Maotai‐flavor liquor;functional prediction;high‐temperature Daqu;Illumina MiSeq high‐throughput sequencing