文章基本信息

标题：Probing the interaction mechanism between Direct Red 80 and Human serum albumin: studies at the molecular level
本地全文：下载
作者：Yan Song ; Yuzhi Shi ; Kejia Zhang 等
期刊名称：E3S Web of Conferences
印刷版ISSN：2267-1242
电子版ISSN：2267-1242
出版年度：2021
卷号：245
页码：3060
DOI：10.1051/e3sconf/202124503060
出版社：EDP Sciences
摘要：In recent years, azo dyes have received increasing attention due to their adverse effects on the environment and consumer health. However, the interaction mechanism between human serum albumin (HSA) and Direct Red 80 (DR80) is still unknown. The results showed that DR80 changed the secondary structure of HSA and made HSA skeleton loose and stretch. In addition, DR80 quenched the endogenous fluorescence of HSA by static quenching and changed the microenvironment of Trp in form of the hydrophobicity increased and the polarity decreased. Molecular docking results indicated that DR80 bound to the interface of three α-helical domains of HSA, hence, the changes in HSA structure and conformation was the main reason for the decline of its esterase activity. This work was done to illuminate the binding mechanism of DR80 and HSA, and to provide a different way for screening the low toxic dye at the molecular level.
其他摘要：In recent years, azo dyes have received increasing attention due to their adverse effects on the environment and consumer health. However, the interaction mechanism between human serum albumin (HSA) and Direct Red 80 (DR80) is still unknown. The results showed that DR80 changed the secondary structure of HSA and made HSA skeleton loose and stretch. In addition, DR80 quenched the endogenous fluorescence of HSA by static quenching and changed the microenvironment of Trp in form of the hydrophobicity increased and the polarity decreased. Molecular docking results indicated that DR80 bound to the interface of three α-helical domains of HSA, hence, the changes in HSA structure and conformation was the main reason for the decline of its esterase activity. This work was done to illuminate the binding mechanism of DR80 and HSA, and to provide a different way for screening the low toxic dye at the molecular level.