文章基本信息

标题：Rapid, Effective, and Versatile Extraction of Gluten in Food with Application on Different Immunological Methods
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作者：Verónica Segura ; Jacobo Díaz ; Ángela Ruiz-Carnicer 等
期刊名称：Foods
电子版ISSN：2304-8158
出版年度：2021
卷号：10
期号：3
页码：652
DOI：10.3390/foods10030652
出版社：MDPI Publishing
摘要：One of the main concerns in gluten analysis is to achieve efficient extraction of gluten proteins. Conventional ethanol-based extraction solutions are inefficient and, because of this, it is necessary to use reducing agents or acids for proper solubilization. The extraction recommended by CODEX Standard 118-1979 (revised 2008) utilizes Cocktail solution (patent WO 02/092633 A1). However, it is harmful with a disgusting odor and is not compatible with some immunological techniques. Here, the versatility and extraction capacity of a new Universal Gluten Extraction Solution (UGES) (patent ES 2 392 412 A1) were evaluated using different methodological conditions, food matrices, and various immunological methods. UGES includes safer compounds for both the user and the environment, and it displayed similar extraction efficiency to that of the extraction method recommended for sandwich enzyme-linked immunosorbent assay (ELISA). The extraction time was significantly reduced from 100 to 40 min, depending on the type of the sample. Furthermore, unlike the currently used solution, UGES is compatible with competitive ELISA.
关键词：celiac disease; gluten proteins; gluten-free diet; gluten extraction solution celiac disease ; gluten proteins ; gluten-free diet ; gluten extraction solution