文章基本信息

标题：Optimal double-spin method for maximizing the concentration of platelets in equine platelet-rich plasma
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作者：Kentaro FUKUDA ; Atsutoshi KUWANO ; Taisuke KURODA 等
期刊名称：Journal of Equine Science
印刷版ISSN：1340-3516
电子版ISSN：1347-7501
出版年度：2020
卷号：31
期号：4
页码：105-111
DOI：10.1294/jes.31.105
出版社：Japanese Society of Equine Science
摘要：This study optimized the double-spin conditions for preparing equine platelet-rich plasma (PRP): leukocyte-rich PRP (L-PRP) and leukocyte-poor PRP (P-PRP). Whole blood samples were centrifuged at various double-spin conditions. Both L-PRP and P-PRP were prepared at each stage, and complete blood counts and growth factor concentrations were compared. Samples centrifuged at 160 × 900 g, 160 × 2,000 g, and 400 × 2,000 g exhibited the highest platelet counts. P-PRP had significantly lower leukocyte and erythrocyte contents than L-PRP, especially at 400 × 2,000 g. No significant differences were observed in growth factor concentrations. Our data suggest that optimum L-PRP preparation should include centrifugation under the aforementioned conditions, whereas centrifugation at 400 × 2,000 g is optimal for P-PRP.
关键词：double-spin;leukocyte;platelet;platelet-rich plasma