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标题：Molecular Detection of Integron in Staphylococcus aureus Isolated From Ruminants
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作者：Amr Rahman ; Helmy Torky ; Sawsan Ebied 等
期刊名称：Alexandria Journal of Veterinary Sciences
印刷版ISSN：1110-2047
电子版ISSN：1110-2047
出版年度：2021
卷号：68
期号：1
页码：90
DOI：10.5455/ajvs.23030
出版社：International Medical Journal Management and Indexing System
摘要：Staphylococcus aureus is one of the most important pathogens of humans and animals, asymptomatically colonizes the anterior nares of humans and animals and it is considered one of the most significant etiological agents of intramammary infection in dairy ruminants, causing both clinical and subclinical mastitis, causing wound infection and dermatitis. A total number of 164 samples (mastitic milk, nasal swabs, wound swabs, abscesses′ contents) were collected from ruminant animals (cattle, sheep and goat) either individually or from farms in Alexandria governorate and subjected to bacteriological examination. Seventeen Staphylococcus aureus isolates were identified morphologically, biochemically. Antibiogram profile of the isolates were carried out against 11 antimicrobials. 16/17 (94%) of isolates showed resistance to at least one antimicrobial, 11/17(64.7) of the isolates showed resistance to three or more of antimicrobials (multidrug resistance), the highest sensitivity was observed for vancomycin and amoxicillin/clavulanic acid 17/17 (100%) while the highest resistance was observed for penicillin 14/17 (82.4%), and gentamicin 9/17(53%). Eight out of seventeen isolates (47%) showed resistance to oxacillin/cefoxitin (methicillin resistant S. aureus) all of them were multi drug resistant (MDR). Ten of MDR S. aureus isolates were molecularly screened for integron. Class 1 integron cassette were detected in 1/10 of tested isolates which exposed to furthersequence analysis revealing dfrA15 gene cassette which encodes trimethoprim resistance. Conclusion: this study report, for the first time, the detection and identification of class 1 integron containing dfrA15 gene in S. aureus isolates which were deposited in the GenBank as Staphylococcus aureus strain AR2020 dihydrofolate reductase 15 (dfrA15) gene with accession number MW036489.
关键词：Staphylococcus aureus; Ruminants; Multi drug resistance; Integron; gene cassette.