文章基本信息

标题：Standardization procedure for flow cytometry data harmonization in prospective multicenter studies
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作者：Lucas Le Lann ; Pierre-Emmanuel Jouve ; Marta Alarcón-Riquelme 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2020
卷号：10
期号：1
页码：1-8
DOI：10.1038/s41598-020-68468-3
出版社：Springer Nature
摘要：One of the most challenging objective for clinical cytometry in prospective multicenter immunomonitoring trials is to compare frequencies, absolute numbers of leukocyte populations and further the mean fluorescence intensities of cell markers, especially when the data are generated from different instruments. Here, we describe an innovative standardization workflow to compare all data to carry out any large-scale, prospective multicentric flow cytometry analysis whatever the duration, the number or type of instruments required for the realization of such projects.