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标题：Connexin43 and connexin50 channels exhibit different permeability to the second messenger inositol triphosphate
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作者：Virginijus Valiunas ; Thomas W. White
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2020
卷号：10
期号：1
页码：1-9
DOI：10.1038/s41598-020-65761-z
出版社：Springer Nature
摘要：Gap junction channels made of different connexins have distinct permeability to second messengers, which could affect many cell processes, including lens epithelial cell division. Here, we have compared the permeability of IP3 and Ca2 through channels made from two connexins, Cx43 and Cx50, that are highly expressed in vertebrate lens epithelial cells. Solute transfer was measured while simultaneously monitoring junctional conductance via dual whole-cell/perforated patch clamp. HeLa cells expressing Cx43 or Cx50 were loaded with Fluo-8, and IP3 or Ca2 were delivered via patch pipette to one cell of a pair, or to a monolayer while fluorescence intensity changes were recorded. Cx43 channels were permeable to IP3 and Ca2 . Conversely, Cx50 channels were impermeable to IP3, while exhibiting high permeation of Ca2 . Reduced Cx50 permeability to IP3 could play a role in regulating cell division and homeostasis in the lens.