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标题：Toxic tall fescue grazing increases susceptibility of the Angus steer fecal microbiota and plasma/urine metabolome to environmental effects
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作者：Ryan S. Mote ; Nicholas S. Hill ; Joseph H. Skarlupka 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2020
卷号：10
期号：1
页码：1-17
DOI：10.1038/s41598-020-59104-1
出版社：Springer Nature
摘要：Impaired thermoregulation and lowered average daily gains (ADG) result when livestock graze toxic endophyte (Epichloë coenophialum)-infected tall fescue (E ) and are hallmark signs of fescue toxicosis (FT), a disease exacerbated by increased temperature and humidity ( temperature-humidity index; THI). We previously reported FT is associated with metabolic and microbiota perturbations under thermoneutral conditions; here, we assessed the influence of E grazing and THI on the microbiota:metabolome interactions. Using high-resolution metabolomics and 16S rRNA gene sequencing, plasma/urine metabolomes and the fecal microbiota of Angus steers grazing non-toxic or E tall fescue were evaluated in the context of THI. E grazing affected the fecal microbiota profile; THI conditions modulated the microbiota only in E steers. E also perturbed many metabolic pathways, namely amino acid and inflammation-related metabolism; THI affected these pathways only in E steers. Integrative analyses revealed the E microbiota correlated and co-varied with the metabolomes in a THI-dependent manner. Operational taxonomic units in the families Peptococcaceae, Clostridiaceae, and Ruminococcaceae correlated with production parameters (e.g., ADG) and with multiple plasma/urine metabolic features, providing putative FT biomarkers and/or targets for the development of FT therapeutics. Overall, this study suggests that E grazing increases Angus steer susceptibility to THI, and offers possible targets for FT interventions.