文章基本信息

标题：Polyfunctional HIV-1 specific response by CD8 T lymphocytes expressing high levels of CD300a
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作者：Joana Vitallé ; Iñigo Terrén ; Leire Gamboa-Urquijo 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2020
卷号：10
期号：1
页码：1-12
DOI：10.1038/s41598-020-63025-4
出版社：Springer Nature
摘要：CD300a receptor is found on different CD8 T cell subsets and its expression has been associated to a more cytotoxic molecular signature. CD300a has an important role in some viral infections and its expression levels are known to be modulated by human immunodeficiency virus (HIV)−1 infection on several cell types. The main objective of this work was to investigate CD300a expression and its regulation during HIV-1 specific CD8 T cell responses. CD300a receptor expression was analysed by multiparametric flow cytometry on CD8 T lymphocytes from HIV negative donors, naive HIV-1 individuals and HIV-1 subjects under suppressive combined antiretroviral therapy (cART). HIV-1 specific CD8 T cell response was studied by stimulating cells with HIV-1 derived peptides or with a Gag HIV-1 peptide. Our results showed that HIV-1 specific CD8 T cells expressing higher levels of CD300a were more polyfunctional showing an increased degranulation and cytokine production. Moreover, we observed an up-regulation of CD300a expression after Gag HIV-1 peptide stimulation. Finally, our results demonstrated an inverse correlation between CD300a expression on CD8 T lymphocytes and HIV disease progression markers. In conclusion, CD300a expression is associated to a better and more polyfunctional HIV-1 specific CD8 T cell response.