首页    期刊浏览 2024年12月15日 星期日
登录注册

文章基本信息

  • 标题:Challenges and opportunities for strain verification by whole-genome sequencing
  • 本地全文:下载
  • 作者:Jenna E. Gallegos ; Sergei Hayrynen ; Neil R. Adames
  • 期刊名称:Scientific Reports
  • 电子版ISSN:2045-2322
  • 出版年度:2020
  • 卷号:10
  • 期号:1
  • 页码:1-9
  • DOI:10.1038/s41598-020-62364-6
  • 出版社:Springer Nature
  • 摘要:Laboratory strains, cell lines, and other genetic materials change hands frequently in the life sciences. Despite evidence that such materials are subject to mix-ups, contamination, and accumulation of secondary mutations, verification of strains and samples is not an established part of many experimental workflows. With the plummeting cost of next generation technologies, it is conceivable that whole genome sequencing (WGS) could be applied to routine strain and sample verification in the future. To demonstrate the need for strain validation by WGS, we sequenced haploid yeast segregants derived from a popular commercial mutant collection and identified several unexpected mutations. We determined that available bioinformatics tools may be ill-suited for verification and highlight the importance of finishing reference genomes for commonly used laboratory strains.
国家哲学社会科学文献中心版权所有