文章基本信息

标题：In vitro and in vivo Anti‐Toxoplasma activity of Dracocephalum kotschyi essential oil
本地全文：下载
作者：Faham Khamesipour ; Seyed Mostafa Razavi ; Seyed Hossein Hejazi 等
期刊名称：Food Science & Nutrition
电子版ISSN：2048-7177
出版年度：2021
卷号：9
期号：1
页码：522-531
DOI：10.1002/fsn3.2021
出版社：John Wiley & Sons, Ltd.
摘要：Toxoplasma gondii is a zoonotic parasite of worldwide importance, responsible for toxoplasmosis in homeotherms. Although treatment options are readily available, most drugs often cause serious side effects. Extracts of Dracocephalum kotschyi ( D. kotschyi ) have shown significant pharmacological activity against various parasites, viruses, and bacteria. In this study, we evaluated the anti‐ T. gondii activity in vitro and in vivo of D. kotschyi essential oil. The thiazolyl blue tetrazolium bromide (MTT) method was used to assess the anti‐ T. gondii activity and cytotoxicity of the essential oil. The presence of T. gondii was observed by Giemsa staining, and the viability was evaluated by the trypan blue staining method. Furthermore, the survival rate of acutely infected mice was evaluated by intraperitoneal injecting of the essential oil (50, 100, and 200 mg kg −1 day −1 ) for five days after infection with 2 × 10 4 tachyzoites. Essential oil, negative, and positive controls that showed the best toxoplasmacidal activity were assayed in triplicate at each concentration. The essential oil exhibited the highest anti‐ Toxoplasma activity with a half‐maximal inhibitory concentration (IC 50 ) of 9.94 ± 0.38 µg, with a selectivity index of 2.463. On Vero cells, the CC 50 of the oil was 24.49 ± 0.96 µg and exhibited a significant anti‐ Toxoplasma activity. Moreover, the treatment by essential oil significantly increased the survival rate compared to untreated infected control. In conclusion, the essential oil might be a useful compound, and with more testing, it may be an excellent alternative to standard chemical drugs in the treatment of toxoplasmosis.
关键词：anti‐T;gondii activity;cytotoxicity; Dracocephalum kotschyi ;essential oil; Toxoplasma gondii