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标题：Suitability of denervated muscle flaps as recipient sites for pancreatic islet cell transplantation
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作者：Jong-Lim Park ; Taewoon Kim ; Baek-Kyu Kim 等
期刊名称：Archives of Plastic Surgery
印刷版ISSN：2234-6163
出版年度：2021
卷号：48
期号：1
页码：133-143
DOI：10.5999/aps.2020.01865
出版社：The Korean Society of Plastic and Reconstructive Surgeons
摘要：Background Extensive research has been conducted on islet transplantation as a possible cure for diabetes. Islet transplantation in the liver via the portal vein has shown remarkable results, but numerous other recipient sites are currently being investigated. We aimed to show the effectiveness of using a muscle flap as a recipient site for islet transplantation. Methods Islet cells were harvested from 12 isogenic Lewis rats, and then diabetes was induced in another 12 isogenic Lewis rats by streptozotocin injection. In six rats, 3,000 islets were transplanted into gastrocnemius muscle flaps, and in the other six rats, the same number of islets were transplanted into the gastrocnemius muscle. The transplanted islet cell function between the two groups was compared by means of blood glucose tests, glucose tolerance tests, immunohistochemistry, and real-time reverse transcription polymerase chain reaction. Results In the muscle flap group, blood glucose levels significantly decreased after islet transplantation. Blood glucose levels were significantly different between the two groups at 3 weeks after transplantation. The muscle flap group showed nearly normoglycemic results upon the glucose tolerance test, whereas the muscle group was hyperglycemic. Immunohistochemical evaluation showed positive results against insulin and glucagon in biopsies of both groups, and the islet cell density was higher in the muscle flap group. There were no statistically significant differences between the two groups in real-time reverse transcription polymerase chain reaction results. Conclusions Our results suggest that muscle flaps are promising candidates for islet cell transplantation.
关键词：Diabetes mellitus; type 1; Islets of Langerhans; Rats; Surgical flaps; Transplantation