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标题：Effects of Vitamin D Supplementation on CD4 T Cell Subsets and mTOR Signaling Pathway in High-Fat-Diet-Induced Obese Mice
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作者：Jeong Hee An ; Da Hye Cho ; Ga Young Lee 等
期刊名称：Nutrients
电子版ISSN：2072-6643
出版年度：2021
卷号：13
期号：3
页码：796
DOI：10.3390/nu13030796
出版社：MDPI Publishing
摘要：Obesity is associated with an impaired balance of CD4 T cell subsets. Both vitamin D and obesity have been reported to affect the mTOR pathway. In this study, we investigated the effects of vitamin D on CD4 T cell subsets and the mTOR pathway. Ten-week-old male C57BL/6 mice were divided into four groups and fed diets with different fat (control or high-fat diets: CON or HFD) and vitamin D contents (vitamin D control or supplemented diets: vDC or vDS) for 12 weeks. T cells purified by negative selection were stimulated with anti-CD3/anti-CD28 mAbs and cultured for 48 h. The percentage of CD4 IL-17 T cells was higher in the vDS than vDC groups. The CD4 CD25 Foxp3 T cells percentage was higher in HFD than CON groups. The phospho-p70S6K/total-p70S6K ratio was lower in vDS than vDC, but the phospho-AKT/total-AKT ratio was higher in vDS than vDC groups. Hif1α mRNA levels were lower in vDS than vDC groups. These findings suggest HIF1α plays an important role in vitamin-D-mediated regulation of glucose metabolism in T cells, and dietary vitamin D supplementation may contribute to the maintenance of immune homeostasis by regulating the mTOR pathway in T cells.
关键词：CD4 T cell; vitamin D supplementation; mTOR pathway; obesity; HIF1α CD4 T cell ; vitamin D supplementation ; mTOR pathway ; obesity ; HIF1α