文章基本信息

标题：Untargeted Metabolomics Analysis of Serum Metabolites in Zucker Diabetic Fatty Rats
本地全文：下载
作者：Qingyuan Liu ; Sze Ying Quach
期刊名称：E3S Web of Conferences
印刷版ISSN：2267-1242
电子版ISSN：2267-1242
出版年度：2021
卷号：233
页码：2017
DOI：10.1051/e3sconf/202123302017
出版社：EDP Sciences
摘要：Zucker Diabtic Fatty rats is a common model used for the studies related to type 2 diabetes. Metabolomics, proven by previous studies, can be used for the prediction of phenotype. This investigation held untargeted metabolomics analysis on the serum metabolites of the Zucker Diabetic Fatty rats, intended to gain insights in the study of the pathology of type 2 diabetes. LCMS method (liquid chromatography coupled with mass spectrometry) is an analytical chemistry technique used to separate and purify complex mixtures. After identifying metabolites using MS2 library spectrum matching, this investigation used Metabo Analyst to compare fatty rats and lean rats. Lastly, by applying over representation analysis, we identified seven metabolic pathways that are important for humans, which assists in discovery of therapeutic targets and the developments of diabetes biomarkers.
其他摘要：Zucker Diabtic Fatty rats is a common model used for the studies related to type 2 diabetes. Metabolomics, proven by previous studies, can be used for the prediction of phenotype. This investigation held untargeted metabolomics analysis on the serum metabolites of the Zucker Diabetic Fatty rats, intended to gain insights in the study of the pathology of type 2 diabetes. LCMS method (liquid chromatography coupled with mass spectrometry) is an analytical chemistry technique used to separate and purify complex mixtures. After identifying metabolites using MS2 library spectrum matching, this investigation used Metabo Analyst to compare fatty rats and lean rats. Lastly, by applying over representation analysis, we identified seven metabolic pathways that are important for humans, which assists in discovery of therapeutic targets and the developments of diabetes biomarkers.