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标题：Studies on the Mycotoxin Detection by an Enzyme Linked Immunosorbent Assay
其他标题：Enzyme Linked Immunosorbent Assay를 이용한 진균독소검출에 관한 연구
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作者：K. Ryeom ; S.J. Yu ; J.H. Lee 等
期刊名称：Environmental Health and Toxicology
电子版ISSN：2233-6567
出版年度：1990
卷号：5
期号：2
语种：English
出版社：Korean Society of Environmental Health and Toxicology
摘要：Aflatoxins,produced by strains of Aspergillus flavus and Aspergillus parasiticus,can be found worldwide in corn,barley,peanuts,and other commodities. Among this group of toxins,aflatoxin Bi was realized to be one of the most potent environmental carcinogens,mutagens and teratogens. It is routinely monitored by methods such as thin layer chromatography,liquid chromatography,fluorodensitometric technique and radioimmunoassay. However,these assays are expensive,necessitate radioactive reagents,and require overnight incubation. In this study,the determination of fungal flora in several sorts cereals has been carried out in order to obtain an appropriate information of the population of fungi. The quantitative analysis of aflatoxin B;has been carried out by High Performance Liquid Chromatography (HPLC) method and Enzyme Linked Immunosorbent Assay (ELISA). The results were summarized as follow: 1) From the 100 samples,313 colonies of fungi were isolated. Among the 313 colonies, 274 were possible to identify into 11 genera. The identified genera were Aspergillus,Penicillium,Mucor,Rhizopus,Alternaria,Cladosorium,Fusarium,Circinella, Chrysosporium,Paecilomyces and Phoma. 2) Six of Aspergillus flawus were aflatoxin- producing strains. Aspergillus flavus isolated from sample barleys was contained the highest content (21.8 u4g/ml) of aflatoxin Br. 3) The yield of aflatoxin B:一oxime compound was appromately 75%. Aflatoxin B: - oxime一Human serum albumin was approved by formal consent as complete antigen. 4) Direct competitive ELISA permitted detection of 0.15 ng levels. In the quantitative microanalysis,ELISA was superior to HPLC method.