文章基本信息

标题：Toxic detection in mine water based on proteomic analysis of lysosomal enzymes in Saccharomyces cerevisiae
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作者：Ngoc-Tu Nguyen ; Yang-Hoon Kim ; Seung Hyuck Bang 等
期刊名称：Environmental Health and Toxicology
电子版ISSN：2233-6567
出版年度：2014
卷号：29
DOI：10.5620/eht.2014.29.e2014019
语种：English
出版社：Korean Society of Environmental Health and Toxicology
摘要：Objectives Lysosome is the cell-organelle which is commonly used as biomonitoring tool in environmental pollution. In this study,the lysosomal proteomic of the yeast Saccharomyces cerevisiae was analyzed for utilization in the detection of toxic substances in mine water samples. Methods This work informs the expression of lysosomal proteomic in yeast in response with toxic chemicals,such as sodium meta-arsenite and tetracycline,for screening specific biomarkers. After that,a recombinant yeast contained this biomarker were constructed for toxic detection in pure toxic chemicals and mine water samples. Results Each chemical had an optimal dose at which the fluorescent protein intensity reached the peak. In the case of water samples,the yeast showed the response with sample 1,3,4,and 5;whereas there is no response with sample 2,6,and 7. Conclusions The recombinant yeast showed a high ability of toxic detection in response with several chemicals such as heavy metals and pharmaceuticals. In the case of mine water samples,the response varied depending on the sample content.
关键词：Heavy metals;Lysosomal proteomic;Lysosomal response;Mine water;Pharmaceuticals