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  • 标题:Toxicity Assessment of Ocean Dumping Wastes Using Fertilization and Embryo Development Rates in the Sea Urchin (Hemicentrotus pulcherrimus)
  • 其他标题:말동성게 (A/em/cenfraft/s pulcherrimus)으\ 수정 및 배 발생률을 이용한 해양배출 폐기물의 독성평가
  • 本地全文:下载
  • 作者:Un-Ki Hwang ; Hyang-Mi Ryu ; Sung-Gil Kim
  • 期刊名称:Environmental Health and Toxicology
  • 电子版ISSN:2233-6567
  • 出版年度:2010
  • 卷号:25
  • 期号:1
  • 语种:English
  • 出版社:Korean Society of Environmental Health and Toxicology
  • 摘要:Toxicity assessment of ocean dumping wastes (dye waste,urban sewage,food waste) were examined in the fertilization and embryo development rates of the Sea Urchin,Hemicentrotus pulcherrimus. Spawning was induced by injecting 1 mL of 0.5 M KCl into coelomic cavity. Males teleased white or cream colored sperms and females released yellow or orange-colored egs. Experiments were began within 30 min the collection of both gametes. The frilization and embryo development rates test were performed for 10 min and 64 h after fertilization,respectively. The frilization and embryo development rates in the control condition (not including ocean dumping wastes sludge elutriate) were greater than 90%,but suddenly decreased with increasing of ocean dumping waste sludge elutriate concentrations. The fertilization and normal embryogenesis rates were significandly inhibited in all waste sludge eluriate from dye waste (ECso=4.37;ECso= 1.76),urban sewage (ECso=5.79;ECso= =2.00) and food waste (ECso=7.68;ECso=2.16),respectively. The NOEC(<3.13) and LOEC(3.13) of frtiliztion and normal embryogenesis rates very similar in all waste sludge elutriate. These results suggest that biological assay using the friliation and embryo development rates o[ H. pulcher- rimus are very useful test method for the ecological loxicity asessment of ocean dumping wastes.
  • 关键词:Hemicentrotus pulcherrimus;frilization rate;embryogenesis;ocean dumping wastes;toxicily
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