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标题：Increased Genotoxicity of N'-methyl-N' nitroguanidine by Oxidative Stress
其他标题：트레스에 의한 AT-methyl-AT-nitroguanidine의 유전독성증가
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作者：Jin Seok Kang ; Ki Kyung Jung ; Soo Kyung Suh 等
期刊名称：Environmental Health and Toxicology
电子版ISSN：2233-6567
出版年度：2007
卷号：22
期号：4
语种：English
出版社：Korean Society of Environmental Health and Toxicology
摘要：To investigate the posible enhancement of genotoxicity in stress environment,we examined the effect of genotoxic material in oxidative stress-induced condition using human cell line. Human lymphoblast cell line. TK6 was treated with hydrogen peroxide (H:O) for induction of oxidative stress,and treated with N-methyI- N'- nitroguanidine (MNNG),as a genetoxic material. We carried out MTS assay to set Ireatment doses. TK6 was treated with H2O: as 6.75 (low dose) or 13.5 μuM (high dose) for 2 h,and treated with MNNG as 0.1 17 (low dose),0.234 (middle dose). 0.468 μM high dose) for 2h. As results,a treatment of MNNG induced DNA dam- age as dose dependently. And TK6 treated with H:O2 at low as well as high dose followed by MNNG treal- ment showed higher DNA damage compared to MNNG alone treated groups. Malondialdehyde,as a marker of lipid peroxidation was increased in H/O;and MNNG treated groups. Real-time RT-PCR analyses for expres- sion of several antioxidative enzymes showed that catalase mRNA and glutathione peroxidase I mRNA expres- sion were decreased in H:Oz and MNNG treated groups. Taken together,we conclude that genotoxicity induced by MNNG is enhanced in a condition of oxidative stress induced by HO: and it suggests that it should be asso- ciated with induction of lipid peroxidation and decrease of antioxidant enzymes.
关键词：genotoxicity;oxidative sress;hydrogen peroxide (H2O2);N'-methyI-N"-nitroguanidine (MNNG); TK6 cell line