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标题：Expression of Metallothionein mRNA in Cadmium Treated Leydig Cells
其他标题：테 스토스테 론생성 레 이 디 히세 포 (Leydig)에 서 의 메탈로치오닌 유전자 발현특성연구
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作者：K wangsik Park
期刊名称：Environmental Health and Toxicology
电子版ISSN：2233-6567
出版年度：2004
卷号：19
期号：3
语种：English
出版社：Korean Society of Environmental Health and Toxicology
摘要：Although the brological functions ot metallothuoneins (MT>) sure stll being Investigated,they have been ugge-ted to be Involved In detoxificaton of heavy metals,(Cdv enging of tree radlcals,and protection against alkylatng agents MTs have been reported to be Induced in most of anmal tssues by heavy metals such dh zinC,copper mercury dand cadm1um,and the proterns hdve binding afinties to the metals However,the presence or inductton of MTs was reported not to be clear 1n leydlg cells which produce testostcrone for the maturation of spermatozoa In male testes In this study. we Investigated the Inducibulty of metallothuonein 1somer\ by cadmium In cultured mouse leydig cells Total RNA was extracted from the near confluent grown leydig cells and RT -PCR was performed using the prumers which were synthesized on the basIs ot MT-I,2. 3 and 4 cDNA from GenBank database As results. MT-1 dand MT-2 mRNA were found to be expressed 1n cadmum non- -treated control cells and MT 1 mRNA expression was dose - dependent when leydig cells were treated w ith cadmum chloride But MT -3 which 1s known to be brain specific and MT-4 whnch 1s another 1soform of metallothtonenn,were not expressed Other genes induced or depressed In cadmum treated leydng cells were also ldentfied by macroarray techniques.
关键词：Cadmum. metallothionern mRNA leydig cells