文章基本信息

标题：A Luminescence-Based Human TRPV1 Assay System for Quantifying Pungency in Spicy Foods
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作者：Minami Matsuyama ; Yuko Terada ; Toyomi Yamazaki-Ito 等
期刊名称：Foods
电子版ISSN：2304-8158
出版年度：2021
卷号：10
期号：1
页码：151
DOI：10.3390/foods10010151
出版社：MDPI Publishing
摘要：The quantitation of pungency is difficult to achieve using sensory tests because of persistence, accumulation, and desensitization to the perception of pungency. Transient receptor vanilloid 1 (TRPV1), which is a chemosensory receptor, plays a pivotal role in the perception of many pungent compounds, suggesting that the activity of this receptor might be useful as an index for pungency evaluation. Although Ca2 -sensitive fluorescence dyes are commonly used for measuring human TRPV1 (hTRPV1) activity, their application is limited, as foods often contain fluorescent substances that interfere with the fluorescent signals. This study aims to design a new pungency evaluation system using hTRPV1. Instead of employing a fluorescent probe as the Ca2 indicator, this assay system uses the luminescent protein aequorin. The luminescence assay successfully evaluated the hTRPV1 activity in foods without purification, even for those containing fluorescent substances. The hTRPV1 activity in food samples correlated strongly with the pungency intensity obtained by the human sensory test. This luminescence-based hTRPV1 assay system will be a powerful tool for objectively quantifying the pungency of spicy foods in both laboratory and industrial settings.
关键词：pungency evaluation; TRPV1; luminescence assay; spicy foods; flavor evaluation pungency evaluation ; TRPV1 ; luminescence assay ; spicy foods ; flavor evaluation