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标题：Deficiency of the RIβ subunit of protein kinase A causes body tremor and impaired fear conditioning memory in rats
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作者：Hieu Hoang Trung ; Toru Yoshihara ; Akito Nakao 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2021
卷号：11
期号：1
页码：2039
DOI：10.1038/s41598-021-81515-x
出版社：Springer Nature
摘要：Abstract The RIβ subunit of cAMP-dependent protein kinase (PKA), encoded by Prkar1b , is a neuronal isoform of the type I regulatory subunit of PKA. Mice lacking the RIβ subunit exhibit normal long-term potentiation (LTP) in the Schaffer collateral pathway of the hippocampus and normal behavior in the open-field and fear conditioning tests. Here, we combined genetic, electrophysiological, and behavioral approaches to demonstrate that the RIβ subunit was involved in body tremor, LTP in the Schaffer collateral pathway, and fear conditioning memory in rats. Genetic analysis of WTC- furue , a mutant strain with spontaneous tremors, revealed a deletion in the Prkar1b gene of the WTC- furue genome. Prkar1b -deficient rats created by the CRISPR/Cas9 system exhibited body tremor. Hippocampal slices from mutant rats showed deficient LTP in the Schaffer collateral–CA1 synapse. Mutant rats also exhibited decreased freezing time following contextual and cued fear conditioning, as well as increased exploratory behavior in the open field. These findings indicate the roles of the RIβ subunit in tremor pathogenesis and contextual and cued fear memory, and suggest that the hippocampal and amygdala roles of this subunit differ between mice and rats and that rats are therefore beneficial for exploring RIβ function.