文章基本信息

标题：食中毒患者，ニワトリおよびウシ由来カンピロバクターのmultiplex PCR binary typing法による型別
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作者：中村寛海 ; 山本香織 ; 藤原淳史等
期刊名称：日本食品微生物学会雑誌
印刷版ISSN：1340-8267
电子版ISSN：1882-5982
出版年度：2020
卷号：37
期号：3
页码：132-142
DOI：10.5803/jsfm.37.132
出版社：Japanese Society of Food Microbiology
摘要：The multiplex PCR binary typing (mP-BIT) is applied to know the relationship among 386 Campylobacter jejuni/coli isolates obtained from patient, bovine, and poultry. As the result of this, two major clusters were observed consist of patient and poultry isolates. One of these clusters contains 27 isolates of patients from 13 cases (11 of 13 cases are poultry related) and 12 poultry isolates. The isolates including this major cluster have 10 genes by mP-BIT. Another cluster contains 19 isolates of patients from 9 cases (7 of 9 cases are poultry related) and 19 poultry isolates. Though little bovine isolates form cluster with patient isolates, major cluster consists only bovine isolates each other. Consequently, human foodborne campylobacteriosis might be mainly related to poultry isolates, on the other hand, only a part of the bovine isolates are related to human foodborne campylobacteriosis. These 386 isolates are also classified by Penner serotyping. As the result of this, Penner O and L are major (both 11 of 159 strains) among patient isolates, Penner D is the major (24 of 91 strains) among bovine isolates, Penner O (11 of 76 strains) is the major among poultry isolates. Though, major isolates (243 of 386; 63%) are Penner serotyping untypeable.
关键词：multiplex PCR binary typing (mP-BIT);Campylobacter;patient;bovine;poultry