文章基本信息

标题：Possibilities of using a miniature fluorescence microscope
本地全文：下载
作者：Alexander Erofeev ; Evgeny Gerasimov ; Dmitry Barinov 等
期刊名称：E3S Web of Conferences
印刷版ISSN：2267-1242
电子版ISSN：2267-1242
出版年度：2020
卷号：224
页码：2005-2014
DOI：10.1051/e3sconf/202022402005
出版社：EDP Sciences
摘要：The article discusses an approach to fluorescence microscopy using single-photon miniature fluorescence microscopes. The use of miniscopes allows you to expand the possibilities of their usage and solve many problems related to the need of fixing experimental material, insufficient scanning speed, complexity and high cost of usage. The authors developed a unique algorithm of correlations building based on cross-correlation of neuronal signals and implemented the opportunity to import experiment’s data in csv format. (as well as a utility for converting data from CNMF-E), which allows to import data from other sources. Special attention is paid to the methods of processing neural activity data: filtering the received frames from noise, compensating of image shifts and distortions.