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标题：Bioavailability and spatial distribution of fatty acids in the rat retina after dietary omega-3 supplementation
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作者：Elisa Vidal ; Bokkyoo Jun ; William C. Gordon 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：2020
卷号：61
期号：12
页码：1733-1746
DOI：10.1194/jlr.RA120001057
出版社：American Society for Biochemistry and Molecular Biology
摘要：Spatial changes of FAs in the retina in response to different dietary n-3 formulations have never been explored, although a diet rich in EPA and DHA is recommended to protect the retina against the effects of aging. In this study, Wistar rats were fed for 8 weeks with balanced diet including either EPA-containing phospholipids (PLs), EPA-containing TGs, DHA-containing PLs, or DHA-containing TGs. Qualitative changes in FA composition of plasma, erythrocytes, and retina were evaluated by gas chromatography-flame ionization detector. Following the different dietary intakes, changes to the quantity and spatial organization of PC and PE species in retina were determined by LC coupled to MS/MS and MALDI coupled to MS imaging. The omega-3 content in the lipids of plasma and erythrocytes suggests that PLs as well as TGs are good omega-3 carriers for retina. However, a significant increase in DHA content in retina was observed, especially molecular species as di-DHA-containing PC and PE, as well as an increase in very long chain PUFAs (more than 28 carbons) following PL-EPA and TG-DHA diets only. All supplemented diets triggered spatial organization changes of DHA in the photoreceptor layer around the optic nerve. Taken together, these findings suggest that dietary omega-3 supplementation can modify the content of FAs in the rat retina.
关键词：diet and dietary lipids ; docosahexaenoic acid ; eicosapentaenoic acid ; lipid biochemistry ; lipid spatial organization ; omega-3 fatty acids ; phosphatidylcholine ; phospholipids ; triglycerides ; very long chain polyunsaturated fatty acids