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标题：A review of phosphatidate phosphatase assays
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作者：Prabuddha Dey ; Gil-Soo Han ; George M. Carman 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：2020
卷号：61
期号：12
页码：1556-1564
DOI：10.1194/jlr.R120001092
出版社：American Society for Biochemistry and Molecular Biology
摘要：Phosphatidate phosphatase (PAP) catalyzes the penultimate step in the synthesis of triacylglycerol and regulates the synthesis of membrane phospholipids. There is much interest in this enzyme because it controls the cellular levels of its substrate, phosphatidate (PA), and product, DAG; defects in the metabolism of these lipid intermediates are the basis for lipid-based diseases such as obesity, lipodystrophy, and inflammation. The measurement of PAP activity is required for studies aimed at understanding its mechanisms of action, how it is regulated, and for screening its activators and/or inhibitors. Enzyme activity is determined through the use of radioactive and nonradioactive assays that measure the product, DAG, or P i . However, sensitivity and ease of use are variable across these methods. This review summarizes approaches to synthesize radioactive PA, to analyze radioactive and nonradioactive products, DAG and P i , and discusses the advantages and disadvantages of each PAP assay.
关键词：Pah1 ; lipin ; enzyme assays ; radioactive assays ; nonradioactive assays ; diacylglycerol ; triacylglycerol ; lipid metabolism