文章基本信息

标题：Emerging frontiers in human milk microbiome research and suggested primers for 16S rRNA gene analysis
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作者：Lilian Lopez Leyva ; Nicholas J.B. Brereton ; Kristine G. Koski 等
期刊名称：Computational and Structural Biotechnology Journal
印刷版ISSN：2001-0370
出版年度：2021
卷号：19
页码：121-133
DOI：10.1016/j.csbj.2020.11.057
出版社：Computational and Structural Biotechnology Journal
摘要：Human milk is the ideal food for infants due to its unique nutritional and immune properties, and more recently human milk has also been recognized as an important source of bacteria for infants. However, a substantial amount of fundamental human milk microbiome information remains unclear, such as the origin, composition and function of the community and its members. There is emerging evidence to suggest that the diversity and composition of the milk microbiome might differ between lactation stages, due to maternal factors and diet, agrarian and urban lifestyles, and geographical location. The evolution of the methods used for studying milk microbiota, transitioning from culture dependent-approaches to include culture-independent approaches, has had an impact on findings and, in particular, primer selection within 16S rRNA gene barcoding studies have led to discrepancies in observed microbiota communities. Here, the current state-of-the-art is reviewed and emerging frontiers essential to improving our understanding of the human milk microbiome are considered.
关键词：Microbiome ; Microbiota ; 16S rRNA gene ; Human Milk ; Microbial barcoding ; PCR primers