文章基本信息

标题：Time-course profiling of bovine alphaherpesvirus 1.1 transcriptome using multiplatform sequencing
其他标题：Time-course profiling of bovine alphaherpesvirus 1.1 transcriptome using multiplatform sequencing
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作者：Norbert Moldován ; Gábor Torma ; Gábor Gulyás 等
期刊名称：Scientific Reports
电子版ISSN：2045-2322
出版年度：2020
卷号：10
期号：1
页码：1-14
DOI：10.1038/s41598-020-77520-1
出版社：Springer Nature
摘要：Long-read sequencing (LRS) has become a standard approach for transcriptome analysis in recent years. Bovine alphaherpesvirus 1 (BoHV-1) is an important pathogen of cattle worldwide. This study reports the profiling of the dynamic lytic transcriptome of BoHV-1 using two long-read sequencing (LRS) techniques, the Oxford Nanopore Technologies MinION, and the LoopSeq synthetic LRS methods, using multiple library preparation protocols. In this work, we annotated viral mRNAs and non-coding transcripts, and a large number of transcript isoforms, including transcription start and end sites, as well as splice variants of BoHV-1. Our analysis demonstrated an extremely complex pattern of transcriptional overlaps.