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标题：Potency Assessment of CBD Oils by Their Effects on Cell Signaling Pathways
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作者：Yasuyo Urasaki ; Cody Beaumont ; Michelle Workman 等
期刊名称：Nutrients
电子版ISSN：2072-6643
出版年度：2020
卷号：12
期号：2
页码：357-373
DOI：10.3390/nu12020357
出版社：MDPI Publishing
摘要：This study used nanofluidic protein posttranslational modification (PTM) profiling to measure the effects of six cannabidiol (CBD) oils and isolated CBD on the signaling pathways of a cultured SH-SY5Y neuronal cell line. Chemical composition analysis revealed that all CBD oils met the label claims and legal regulatory limit regarding the CBD and tetrahydrocannabinol (THC) contents, respectively. Isolated CBD was cytotoxic, with an effective concentration (EC50) of 40 µM. In contrast, the CBD oils had no effect on cell viability at CBD concentrations exceeding 1.2 mM. Interestingly, only an unadulterated CBD oil had strong and statistically significant suppressive effects on the pI3K/Akt/mTOR signaling pathway with an EC50 value of 143 µM and a slow-acting timescale requiring hours. Systematic profiling of twenty-six proteins, which served as biomarkers for nine signaling pathways, revealed that the unadulterated CBD oil downregulated seven signaling pathways but had no measurable effect on the other two signaling pathways. The remaining CBD oils, which were adulterated, and isolated CBD had weak, variable, or undetectable effects on neuronal signaling pathways. Our data clearly showed that adulteration diminished the biological activities of CBD oils. In addition, nanofluidic protein PTM profiling provided a robust means for potency assessment of CBD oils.
关键词：cannabidiol; protein posttranslational modification; nanofluidic proteomics; neuronal signaling pathways; adulteration; potency assessment; capillary isoelectric focusing cannabidiol ; protein posttranslational modification ; nanofluidic proteomics ; neuronal signaling pathways ; adulteration ; potency assessment ; capillary isoelectric focusing