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标题：Anti-Obesity Activities of Chikusetsusaponin IVa and Dolichos lablab L. Seeds
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作者：Jun Yin ; Chang-Seob Seo ; In Hyeok Hwang 等
期刊名称：Nutrients
电子版ISSN：2072-6643
出版年度：2018
卷号：10
期号：9
页码：1221-1232
DOI：10.3390/nu10091221
出版社：MDPI Publishing
摘要：Obesity, a condition where excess body fat accumulates to the extent, causes a negative effect on health. Previously, we reported the extract of Dolichos lablab L. (DLL-Ex) inhibited high-fat diet (HFD)-induced increases in body weight and body fat mass and ameliorated increases in body weight. In the present work, we studyed the molecular mechanism for the inhibitory effect of DLL-Ex or Chikusetsusaponin IVa (CS-IVa), as isolated from Dolichos lablab L. (DLL) seeds extract, on adipocyte differentiation. We evaluated the effect of DLL-Ex, an anti-obesity agent, and CS-IVa, an active component of DLL-Ex, on 3T3-L1 cell differentiation via Oil red O assay and Q-PCR, along with their effects on CCAAT element binding protein alpha (C/EBPα), peroxisome proliferator-activated receptor gamma (PPARγ), fatty acid synthase (FAS), and fatty acid-binding protein 4 (FABP4) mRNA transcriptions. FAS and FABP4 protein expression levels after exposure to CS-IVa were also tested. The results showed that DLL-Ex and CS-IVa have potent inhibitory activity on adipocyte differentiation. Therefore, DLL and CS-IVa may be developed as a functional food material to treat obesity.
关键词：Dolichos lablab L;; chikusetsusaponin IVa; triterpenoid saponin; anti-obesity; 3T3-L1 cell differentiation Dolichos lablab L; ; chikusetsusaponin IVa ; triterpenoid saponin ; anti-obesity ; 3T3-L1 cell differentiation