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标题：Cocoa Diet Prevents Antibody Synthesis and Modifies Lymph Node Composition and Functionality in a Rat Oral Sensitization Model
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作者：Mariona Camps-Bossacoma ; Mar Abril-Gil ; Sandra Saldaña-Ruiz 等
期刊名称：Nutrients
电子版ISSN：2072-6643
出版年度：2016
卷号：8
期号：4
页码：242-258
DOI：10.3390/nu8040242
出版社：MDPI Publishing
摘要：Cocoa powder, a rich source of polyphenols, has shown immunomodulatory properties in both the intestinal and systemic immune compartments of rats. The aim of the current study was to establish the effect of a cocoa diet in a rat oral sensitization model and also to gain insight into the mesenteric lymph nodes (MLN) activities induced by this diet. To achieve this, three-week-old Lewis rats were fed either a standard diet or a diet with 10% cocoa and were orally sensitized with ovalbumin (OVA) and with cholera toxin as a mucosal adjuvant. Specific antibodies were quantified, and lymphocyte composition, gene expression, and cytokine release were established in MLN. The development of anti-OVA antibodies was almost totally prevented in cocoa-fed rats. In addition, this diet increased the proportion of TCRγδ and CD103 CD8 cells and decreased the proportion of CD62L CD4 and CD62L CD8 cells in MLN, whereas it upregulated the gene expression of OX40L, CD11c, and IL-1β and downregulated the gene expression of IL-17α. In conclusion, the cocoa diet induced tolerance in an oral sensitization model accompanied by changes in MLN that could contribute to this effect, suggesting its potential implication in the prevention of food allergies.
关键词：cholera toxin; flavonoids; intestinal sensitization; nutraceutic; oral tolerance; ovalbumin; specific antibodies; Tγδ cells cholera toxin ; flavonoids ; intestinal sensitization ; nutraceutic ; oral tolerance ; ovalbumin ; specific antibodies ; Tγδ cells