文章基本信息

标题：Activity-Guided Fractionation of Red Fruit Extracts for the Identification of Compounds Influencing Glucose Metabolism
本地全文：下载
作者：Johanna Josefine Ostberg-Potthoff ; Kirsten Berger ; Elke Richling 等
期刊名称：Nutrients
电子版ISSN：2072-6643
出版年度：2019
卷号：11
期号：5
页码：1166-1186
DOI：10.3390/nu11051166
出版社：MDPI Publishing
摘要：An activity-guided search for compounds influencing glucose metabolism in extracts from aronia (Aronia melanocarpa, A.), pomegranate (Punica granatum L., P.), and red grape (Vitis vinifera, RG) was carried out. The three extracts were fractionated by means of membrane chromatography to separate the anthocyanins from other noncolored phenolic compounds (copigments). In addition, precipitation with hexane was performed to isolate the polymers (PF). The anthocyanin and copigment fractions (AF, CF) of aronia, pomegranate, and red grape were furthermore fractionated with high-performance countercurrent chromatography (HPCCC) and the subfractions were characterized by HPLC-PDA-MS/MS analyses. Each of the (sub-)fractions was examined by in vitro-tests, i.e., the inhibition of the activity of α-amylase and α-glucosidase. On the basis of this screening, several potent inhibitors of the two enzymes could be identified, which included flavonols (e.g., quercetin), ellagitannins (e.g., pedunculagin), and anthocyanins (e.g., delphinidin-3-glucoside and petunidin-3-glucoside). In the α-glucosidase assay all of the examined fractions and subfractions of the fruit extracts were more active than the positive control acarbose.
关键词：aronia; pomegranate; red grape; polyphenols; HPLC-PDA-MS/MS; α-amylase; α-glucosidase; high-performance countercurrent chromatography (HPCCC) aronia ; pomegranate ; red grape ; polyphenols ; HPLC-PDA-MS/MS ; α-amylase ; α-glucosidase ; high-performance countercurrent chromatography (HPCCC)