文章基本信息

标题：Development of Lateral Flow Immunochromatographic Assays Using Colloidal Au Sphere and Nanorods as Signal Marker for the Determination of Zearalenone in Cereals
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作者：Mingfei Pan ; Tianyu Ma ; Jingying Yang 等
期刊名称：Foods
电子版ISSN：2304-8158
出版年度：2020
卷号：9
期号：3
页码：281-291
DOI：10.3390/foods9030281
出版社：MDPI Publishing
摘要：This paper describes the development of lateral flow immunochromatographic assays (ICAs) using colloidal Au sphere (SP) and nanorods (NRs) as signal markers for the determination of zearalenone (ZEN) in cereals. The developed ICAs can detect the analyte ZEN within a short time (10 min), and achieve lower limit of detection (LOD). This is the first time that the AuNRs are used as signal probe in immune test strip for ZEN detection. For colloidal AuSP immunochromatographic analysis (AuSP-ICA), the LODs in solution and spiked cereal sample were 5.0 μg L−1 and 60 μg kg−1, and for AuNRs immunochromatographic analysis (AuNRs-ICA) the two LODs achieved 3.0 μg L−1 and 40 μg kg−1, respectively. These two proposed ICAs have minor cross-reaction to the structural analogs of ZEN, and no cross-reactivity with aflatoxin B1, T-2 toxin, ochratoxin A, deoxynivalenol, fumonisin B1. Both of the developed ICAs can specifically and sensitively detect ZEN in cereals, providing an effective strategy for rapid screening and detection of ZEN in a large number of food samples.
关键词：zearalenone; immunochromatographic assay; colloidal Au sphere; Au nanorods; cereals zearalenone ; immunochromatographic assay ; colloidal Au sphere ; Au nanorods ; cereals